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KMID : 1036720160490010059
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Journal of Nutrition and Health
2016 Volume.49 No. 1 p.59 ~ p.62
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Role of zinc for calcification inhibitor protein in vascular smooth muscle cell plaque formation
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신미영:Shin Mee-Young
권인숙:Kwun In-Sook
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Abstract
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Purpose: Zinc, a biomineral present within and outside cells, manages various cellular mechanisms. In this study, we
examined whether zinc was involved in vascular smooth muscle cell (VSMC) calcification via regulation of calcification
inhibitor protein, osteopontin (OPN).
Methods: Rat aorta cell line (A7r5 cells) and primary vascular smooth muscle cells (pVSMCs) from rat aorta were cultured with phosphate (1-5 mM) and zinc (0-15 μM) as appropriate, along with osteoblasts (MC3T3-E1) as control. The cells were then stained for Ca and P deposition for calcification examination as well as osteopontin expression as calcification inhibitor protein was measured.
Results: Both Ca and phosphate deposition increased as the addition of phosphate increased. In the same manner, the expression of osteopontin was upregulated as the addition of phosphate increased in both cell types. When zinc was added, Ca and P deposition decreased in VSMCs, while it increased in osteoblasts.
Conclusion: The results imply that zinc may prevent VSMC calcification by stimulating calcification inhibitor protein OPN synthesis in VSMCs.
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KEYWORD
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vascular smooth muscle cells, calcification, osteopontin, osteoblasts
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